Escherichia coli expression system for producing mature human tyrosinase and a producing method thereof

Abstract

An E. coli expression system for producing mature human tyrosinase is provided and includes an E. coli host, which has a trait for expressing endogenous methionyl aminopeptidase in cytoplasm, and an expression vector transformed into the E. coli host. The expression vector has a replication origin sequence of an E. coli, and the expression vector includes an inducible promoter and a DNA fragment of human tyrosinase having a sequence referenced as SEQ ID NO:3, wherein a 5′ end of the DNA fragment is constructed at a restriction enzyme NdeI recognition site located at the downstream of the inducible promoter.

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