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AssigneeSan Diego State University Research Foundation
InventorsKaren Diane MAY-NEWMAN, Irwin Hai-Wen LING, John Nicholas SAM-SOON, York Kin WONG
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The invention provides a unique cell culture chamber, as well as systems for culturing and analyzing cells, methods of using the chamber, and techniques for culturing and analyzing cells. The invention also provides an apparatus for distributing cells across a substrate, comprising a chamber comprised of at least one polydimethylsiloxane (PDMS) surface, where the chamber comprises one or more wells, and each well can contain a volume of a suspension of cells in culture, and where the chamber, when placed over a substrate, provides a more uniform dispersement of cells on the substrate than when the cells are deposited on a substrate without said chamber.
An apparatus for distributing cells across a surface, comprising a chamber comprised of at least one polydimethylsiloxane (PDMS) surface, wherein the chamber comprises one or more depressions, wherein the depressions can contain a volume of a suspension of cells in culture, and wherein the chamber, when placed over a substrate, provides a more uniform dispersement of cells on the substrate than when the cells are deposited on a substrate without said chamber.
A method for the uniform distribution of cells across a substrate, comprising,
A cell culture apparatus, comprising a polydimethylsiloxane chamber and a substrate, wherein polydimethylsiloxane chamber attaches to the substrate, and wherein the polydimethylsiloxane surface 1) reduces cell aggregation on the substrate, 2) increases cell adhesion to the substrate, 3) provides a more uniform dispersement of cells across the substrate, or a combination thereof, as compared to a cell culture apparatus not containing a polydimethylsiloxane chamber.
Dependencies are inferred from the claim text.