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US 2013/0280714 A1Published application
Rapid salmonella serotyping assay
AssigneeCenters for Disease Control and Prevention
InventorsPatricia Fields, John R. McQuiston, Collette Fitzgerald Leaumont
Life of this patent
Estimating the term…
Abstract
Processes for the serotype specific detection and identification of one or more Salmonella serotypes are provided. A family of specific primers and probes are provided that allow screening of biological or environmental samples for robust, rapid, and reproducible detection and identification of one or more Salmonella serotypes in the sample.
Claims16
A process of serotyping Salmonella in a sample comprising:
- adding to a sample one or more O-grp-2 primer sets operable to produce an O-grp-2 amplification product of an O-grp-2 target sequence;
- said primer set comprising a forward primer and a reverse primer that hybridize to distinct regions flanking said O-grp-2 target sequence under conditions suitable for a polymerase chain reaction;
- and
- detecting the presence or absence of said O-grp-2 amplification product by hybridization of one or more labeled probes specific to said O-grp-2 amplification product, said labeled probe comprising a label and a nucleotide sequence, said nucleotide sequence comprising the sequence of SEQ ID NO:
- 131, 132, 133, 134 or 135.
2 more elements
A probe for detection of a nucleotide sequence in a sample comprising
- an isolated nucleotide sequence consisting of the sequence of SEQ ID NO:
- 131, 132, 133, 134 or 135;
- and
- a label.
1 more element
A kit for serotyping Salmonella in a sample comprising:
- a probe of 20 to 40 nucleotides, said probe capable of hybridizing to an O-grp-2 amplicon.
Dependencies are inferred from the claim text.