Probe, and Polymorphism Detection Method Using the Same
AssigneeARKRAY, Inc.
InventorMoeko Ijuin
Life of this patent
Estimating the term…
Abstract
The present invention provides a probe for detecting a V600 polymorphism in the BRAF gene, which is (P1) a fluorescently labeled oligonucleotide which has an identity of at least 80% to a base sequence having a length of 10 to 50 bases including the 228th to the 237th bases of the base sequence indicated in SEQ ID NO:1, wherein the base corresponding to the 237th base is cytosine labeled with a fluorescent dye, the oligonucleotide recognizing a polymorphism in at least one of the 228th to the 230th bases of the base sequence indicated in SEQ ID NO:1 (with the proviso that the oligonucleotide is not the one indicated in SEQ ID NO:7 or 19).
Claims18
A probe for detecting a V600 polymorphism in a BRAF gene, the probe comprising the following P1 fluorescently labeled oligonucleotide:
- (P1) an oligonucleotide having an identity of at least 80% with respect to a base sequence having a length of 10 to 50 bases including the 228th to the 237th bases of the base sequence indicated in SEQ ID NO:1, wherein the base corresponding to the 237th base is a cytosine labeled with a fluorescent dye, and the oligonucleotide recognizes a polymorphism in at least one of the 228th to the 230th bases of the base sequence indicated in SEQ ID NO:1, with the proviso that the oligonucleotide is not an oligonucleotide with a sequence as set forth in SEQ ID NO:7 or 19.
(canceled)
Dependencies are inferred from the claim text.