US 2013/0280697 A1 · HEV Assay

Abstract

A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single none-degenerate forward primer partially overlapping the 5′UTR region of HEV and at least one reverse primer. Also disclosed are related methods comprising a probe, and kits for the detection of genotypes 1, 2, 3 and/or 4 of HEV.

Claims17

Dependent claims

A method for simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV if present in a biological sample, comprising the steps of:

(a) isolating nucleic acids present in the biological sample;
(b) amplifying the nucleic acids isolated in step (a) using one non-degenerate forward primer and at least one non-degenerate reverse primer, wherein the forward and reverse primers are capable of simultaneously amplifying genotypes 1, 2, 3, and/or 4 of HEV.

Dependencies are inferred from the claim text.

Facts

Application

13/864,439Filed 17 Apr 2013

Priority

18 Apr 2012

Published

24 Oct 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification3 codes

C12Q 1/707
For hepatitis: non-A, non-B Hepatitis, excluding hepatitis D
C12Q 2600/16
Oligonucleotides characterized by their use: primer sets for multiplex assays
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

HEV Assay

Life of this patent

HEV Assay

Life of this patent

Abstract

Claims17