Humanized Antibodies with Anti-Tumor Activity
AssigneeCephalon Australia Pty Ltd
InventorsAdam W. Clarke, Anthony G. Doyle, Philip A. Jennings, Norbert Kienzle, Matthew Pollard
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Abstract
The present invention provides humanised antibodies and binding domains thereof with anti-tumour activity. In particular the humanised antibodies have specific binding to and direct killing of human colon tumour cells and display potent immune-mediated cytotoxic activity against human colon cancer cells in vitro using antibody-dependent cell-mediated cytotoxicity (ADCC) and complement dependent cytotoxicity (CDC) assays and in vivo using mouse tumour models.
Claims37
1-52. (canceled)
A humanized antibody, comprising a VH binding domain having an amino acid sequence selected from the group consisting of the amino acid sequence of residues 1 to 120 of SEQ ID NO:
- 50, the amino acid sequence of residues 1 to 120 of SEQ ID NO:
- 38, an amino acid sequence having at least 95% identity with the amino acid sequence of residues 1 to 120 of SEQ ID NO:
- 50 provided that the residue at position 72 is Arg or Lys, and an amino acid sequence having at least 95% identity with the amino acid sequence of residues 1 to 120 of SEQ ID NO:
- 38 provided that the residue at position 72 is Arg or Lys, and a VL binding domain having an amino acid sequence selected from the group consisting of the amino acid sequence of residues 1 to 107 of SEQ ID NO:
- 7, the amino acid sequence of residues 1 to 107 of SEQ ID NO:
- 8, an amino acid sequence having at least 95% identity with the amino acid sequence of residues 1 to 107 of SEQ ID NO:
- 7, and an amino acid sequence having at least 95% identity with the amino acid sequence of residues 1 to 107 of SEQ ID NO:
- 8.
A humanized antibody, comprising a light chain comprising the amino acid sequence of SEQ ID NO:
- 7 and a heavy chain comprising the amino acid sequence of SEQ ID NO:50.
A humanized antibody, comprising a light chain comprising the amino acid sequence of SEQ ID NO:
- 8 and a heavy chain comprising the amino acid sequence of SEQ ID NO:38.
Dependencies are inferred from the claim text.