Materials and Methods for Improved Immunoglycoproteins
AssigneeEmergent Product Development Seattle, LLC
InventorsPeter Robert Baum, Erik Stephen Espling, Peter Armstrong Thompson
Life of this patent
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Abstract
Immunoglycoproteins, including antibodies, with improved ADCC and altered glycosylation patterns arc provided. Also provided are cell culturing methods and media for producing such immunoglycoproteins, and therapeutic uses of such immunoglycoproteins.
Claims15
A method for increasing the antibody-dependent cytoxicity (ADCC) of immunoglycoprotein molecules produced by a host cell, comprising the step of:
- growing said host cell in a volume of at least 1 liter of culture medium comprising castanospermine at a concentration between about 25 and about 800 μM that increases the ADCC of immunoglycoprotein molecules produced by said host cell.
A method for increasing the CD 16 binding of immunoglycoprotein molecules produced by a host cell, comprising the step of:
- growing said host cell in a volume of at least 1 liter of culture medium comprising castanospermine at a concentration between about 25 and about 800 μM that increases the CD16 binding of immunoglycoprotein molecules produced by said host cell.
The method of any one of claims 1-7 wherein the level of immunoglycoprotein production in the culture medium is at least 100 μg/mL.
Dependencies are inferred from the claim text.