Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of aspartyl-trna synthetases

A therapeutic composition, comprising an isolated aminoacyl-tRNA synthetase (AARS) polypeptide of about 100 to about 750 amino acids in length and comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 98%, or 100% identical to a sequence in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, wherein the polypeptide has a solubility of at least about 5 mg/mL, and wherein the composition has a purity of at least about 95% on a protein basis and less than about 10 EU endotoxin/mg protein.

An isolated aminoacyl-tRNA synthetase (AARS) polypeptide of about 100 to about 750 amino acids in length and comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 98%, or 100% identical to a sequence in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9.

A method of determining presence or levels of an AARS polypeptide in a sample, comprising contacting the sample with one or more binding agents that specifically bind to an AARS polypeptide as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, detecting the presence or absence of the binding agent, and thereby determining the presence or levels of the AARS polypeptide.

An engineered full length aminoacyl-tRNA synthetase (AARS) protein comprising a heterologous proteolysis site to enable the proteolytic generation of an AARS polypeptide as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9.

A composition, comprising a binding agent that specifically binds to an isolated aminoacyl-tRNA synthetase (AARS) polypeptide fragment as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, wherein the binding agent has an affinity of at least about 1 nM for the polypeptide fragment.