Methods of obtaining cell populations enriched with desired cells

A selection method, comprising:

• providing a population of cells comprising a target type of differentiated cells having a pre-identified cytoskeletal profile and at least one cell selected from the group consisting of undifferentiated cells, differentiating cells and differentiated cells being different from the target type of differentiated cells;
• and
• incubating the population of cells with a cytotoxic agent selective to a pre-identified cytoskeletal profile characterizing the target type of differentiated cells, in an amount and for a time period effective to selectively provide a modified population of cells comprising predominantly or consisting essentially of the target type of differentiated cells which do not develop rosette structures.

A cell culture consisting of a target type of differentiated cells having a pre-identified cytoskeletal profile and that do not develop rosette structures, the target type of differentiated cells produced by in vitro treatment with a cytotoxic agent selective to the pre-identified cytoskeletal profile, a population of cells comprising the target type of differentiated cells and at least one cell selected from the group consisting of undifferentiated cells, differentiating cells and differentiated cells being different from the target type of differentiated cells.

A cell culture consisting of a target type of differentiated cells having a pre-identified cytoskeletal profile and a cytotoxic agent selective to the pre-identified cytoskeletal profile characterizing the target type of differentiated cells, the target type of differentiated cells being resistant to said cytotoxic agent.

A method comprising transplantation of a composition of cells comprising predominantly or consisting essentially of a target type of differentiated cells having a pre-identified cytoskeletal profile and which do not develop rosette structures, the target type of differentiated cells being produced by in vitro treatment with a cytotoxic agent selective to the pre-identified cytoskeletal profile, a population of cells comprising the target type of differentiated cells and at least one cell selected from the group consisting of undifferentiated cells, differentiating cells and differentiated cells being different from the target type of differentiated cells.