Sub-2 micron chiral stationary phase separation agents for use with supercritical fluid chromatography

A chiral separation column for use with supercritical fluid chromatography wherein the chiral separation column contains a chiral stationary phase agent which is coated or covalently bonded and has a particle size less than about 2 microns in diameter, said chiral stationary phase agent comprising a porous granular carrier and a polysaccharide or derivatized polysaccharide, wherein said porous granular carrier is porous having a particle size between about 1.5 and about 1.9 microns and having an average pore size of from about 50 Angstroms to about 200 Angstroms and that said porous granular carrier has a ratio of pore size/particle size from about 0.0026 to about 0.0133, wherein the porous granular carrier is coated with the polysaccharide or derivatized polysaccharide or wherein the porous granular carrier is covalently bonded to the polysaccharide or derivatized polysaccharide, and wherein the polysaccharide or derivatized polysaccharide is selected from the group consisting of cellulose tris-(3,5-dimethylphenylcarbamate), cellulose tris-(3-chloro-4-methylphenylcarbamate), amylose tris-(3,5-dimethylphenylcarbamate), amylose tris-(3-chloro-4-methylphenylcarbamate), cellulose tris-(4-methylbenzoate), amylose tris-(4-methylbenzoate), amylose tris-(4-chloro-3-methylphenylcarbamate), amylose tris-(5-chloro-2-methylphenylcarbamate), cellulose tris-(4-chloro-3-methylphenylcarbamate), and cellulose tris-(5-chloro-2-methylphenylcarbamate), and wherein the porous granular carrier is selected from the group consisting of silica, alumina, magnesia, titanium oxide, glass, silicate, and kaolin.