Back to results
US 2013/0274146 A1Published application
Capture reactions
AssigneeGood Start Genetics, Inc.
InventorsMark Umbarger, Gregory Porreca, Charles Towne, George Church
Life of this patent
Estimating the term…
Abstract
The invention generally relates to methods of performing a capture reaction. In certain embodiments, the method involves obtaining a nucleic acid, fragmenting the nucleic acid, and capturing a target sequence on the nucleic acid fragment using a capture moiety, such as a molecular inversion probe.
Claims9
A method of improving performance of molecular inversion probe capture reactions, the method comprising:
- obtaining a nucleic acid sample;
- fragmenting the nucleic acid into nucleic acid fragments;
- introducing a plurality of molecular inversion probes into the sample, wherein each probe is specific for a different target and each probe comprises a first targeting arm and a second targeting arm joined to a universal central region;
- capturing multiple targets within the sample with two or more of the molecular inversion probes, the multiple targets being on one or more of the nucleic acid fragments;
- and
- amplifying two or more of the targets in a single reaction vessel.
3 more elements
Dependencies are inferred from the claim text.