Loading patent…
AssigneeNational University of Ireland,Galway a university
InventorsKate Mary Reddington, Thomas Gerard Barry, Justin Joseph O'Grady, Terence James Smith
Estimating the term…
The present invention relates to a multiplex in vitro nucleic acid amplification method for identifying a species of the Mycobacterium tuberculosis complex present in a sample. The method includes detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex. The invention also includes kits containing primers or probes for conducting this method, nucleic acids useful for performing this method and diagnostic techniques using these nucleic acids.
-136. (canceled)
A multiplex in vitro nucleic acid amplification method for identifying which specific species of the Mycobacterium tuberculosis complex is present in a sample, wherein the method comprises detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample, wherein the nucleic acid molecule targets are present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex, and wherein the method distinguishes between the following Mycobacterium tuberculosis complex species:
A multiplex in vitro nucleic acid amplification method for identifying a species of the Mycobacterium tuberculosis complex present in a sample, wherein the method comprises detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex.
The method of any preceding claim comprising the use of primers or probes specific for lepA, SEQ ID NO:
A kit comprising a set of primers and probes which are specific for a plurality of nucleic acid molecule targets, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex;
A diagnostic technique for identifying a species of the Mycobacterium tuberculosis complex comprising hybridising sample nucleic acid molecules to one or more nucleic acid molecules which comprise a sequence selected from the group consisting of SEQ ID NOs:
Dependencies are inferred from the claim text.