Methods for increasing microbial production of isoprene, isoprenoids, and isoprenoid precursor molecules using glucose and acetate co-metabolism
AssigneeDanisco US Inc.
InventorsGopal K. CHOTANI, Alex T. NIELSEN, Dmitrii V. VAVILINE
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Abstract
Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels.
Claims33
A method for improving the efficiency of the production of isoprene by recombinant host cells in culture, the method comprising culturing said recombinant host cells in culture media comprising a carbon source and acetate under suitable conditions for the production of isoprene,
- wherein said recombinant host cells comprise one or more heterologous nucleic acids encoding an isoprene synthase polypeptide;
- wherein said recombinant host cells are capable of producing isoprene;
- and
- wherein isoprene production by said recombinant host cells cultured in the culture media comprising a carbon source and acetate is improved compared to the isoprene production by said recombinant host cells cultured in culture media comprising a carbon source in the absence of acetate.
A method for improving the efficiency of the production of isoprenoid precursors by a recombinant host cell, the method comprising:
- a. providing one or more recombinant host cells comprising one or more heterologous nucleic acids encoding one or more MVA pathway polypeptides;
- and
- b. culturing said recombinant host cells in the presence of glucose and acetate under suitable conditions for the production of isoprenoid precursors;
- wherein the production of isoprenoid precursors by said recombinant host cells cultured in the presence of glucose and acetate is improved compared to the production of isoprenoid precursors by said recombinant host cells cultured in the presence of glucose alone.
A method for improving the efficiency of the production of isopreniods by recombinant host cells in culture, the method comprising culturing said recombinant host cells in culture media comprising a carbon source and acetate under suitable conditions for the production of isopreniods,
- wherein said recombinant host cells comprise (i) one or more heterologous nucleic acid encoding the entire MVA pathway and (ii) one or more heterologous nucleic acids encoding for a polyprenyl pyrophosphate synthase;
- wherein said recombinant host cells are capable of producing an isoprenoid;
- and
- wherein isoprenoid production by said recombinant host cells cultured in the presence of glucose and acetate is improved compared to the isoprenoid production by said recombinant host cells cultured in the presence of glucose alone.
Dependencies are inferred from the claim text.