Loading patent…
AssigneeCj Cheiljedang Corporation
InventorsSo Young Kim, Yong Uk Shin, Chang Il Seo, Sung Kwang Son, In Kyung Heo, Han Jin Lee, Ju Eun Kim, Hyun Ah Kim, Jee Yeon Bae, Kwang Ho Na
Estimating the term…
The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.
An isolated protein having O-acetylhomoserine sulfhydrylase activity, represented by the amino acid sequence of SEQ ID No. 13.
A mutant protein having enhanced O-acetylhomoserine sulfhydrylase activity than a wild type, wherein a 3rd amino acid from the N-terminal of the protein represented by the amino acid sequence of SEQ ID No. 13 is substituted with an amino acid other than isoleucine, a 65th amino acid from the same is substituted with an amino acid other than phenylalanine, a 104th amino acid from the same is substituted with an amino acid other than valine, or one or more of the three types of amino acid mutations is made.
Dependencies are inferred from the claim text.