Back to results
US 2013/0260467 A1Published application
Molecular delivery with nanowires
AssigneePresident and Fellows of Harvard
InventorsHongkun Park, Jacob Robinson, Amy Sutton, Marsela Jorgolli, Alexander K. Shalek
Life of this patent
Estimating the term…
Abstract
A molecular delivery device including a plurality of nanowires (e.g., Si NWs) coated with an electrically conductive layer. Also disclosed are methods for delivering a molecule by nanowire-mediated electroporation.
Claims28
A molecular delivery device comprising:
- a substrate having a surface, and
- a plurality of nanowires attached to the surface;
- wherein the substrate is electrically conductive, and the nanowires are coated with an electrically conductive layer.
A method of delivering a molecule to a cell, the method comprising:
- providing a substrate having a surface and a plurality of nanowires attached to the surface, wherein both the substrate and nanowires are electrically conductive;
- contacting the nanowires with the cell to allow penetration of one or more nanowires into the cell, wherein the cell is immersed in a bath solution containing the molecule;
- and
- applying a current or voltage waveform between the substrate and an electrode in the bath solution, whereby the molecule enters into the cell through transiently formed pores on cell membrane.
1 more element
A method of delivering a molecule to a cell, the method comprising:
- providing a substrate having a surface and a plurality of electrically conductive nanowires, each of which has a first end and a second end, wherein the substrate and each of the nanowires are coated with an electrically insulating layer except for the first and second ends of each of the nanowires, the first end is attached to the surface, and the second end is coated with an electrically conductive layer;
- contacting the nanowires with the cell immersed in a bath solution containing the molecule to allow penetration of one or more nanowires into the cell;
- and
- applying a current or voltage waveform between two electrodes, one connected to the first end of each of the nanowires and the other placed in the bath solution, whereby the molecule enters into the cell through transiently formed pores on cell membrane.
1 more element
Dependencies are inferred from the claim text.