Method for the purification and stabilisation of enzyme gluconate dehydrogenase (gadh, ec 1.1.99.3), enzyme gluconate dehydrogenase (gadh, ec 1.1.99.3), and the use of enzyme gluconate dehydrogenase (gadh, ec 1.1.99.3)

Process of purification and stabilization of the enzyme Gluconate Dehydrogenase (GADH, EC 1.1.99.3) either recombinant or not, in which the purification of GADH is carried out from cells of Serratia marcescens, Kleibsella pneumoniae, Pseudomones aeruginosa, Pseudomonas fluorescens, Gluconobacter oxydans, Gluconobacter industrius or Eschericia coli or a mixture of them, and in which the cellular breakage is produced by sonication of another type of physical breakage, then obtaining their membranes, characterized in that:

• a) the membranes are resuspended by extrusion and
• b) the solubilization of the enzyme GADH of the membrane is realized by the addition of detergents such as n-Octyl-β-D-thioglucoside, Zwittergent 342, Twenn 80, Brij 58 or Triton X-100 in v/v percentages between 0.1% and 3%, the suspension is ultracentrifugated and the supernatant was collected,
• c) the supernatant is subjected to a chromatography of ion exchange at a pH between 4 and 8.5.