Activating phosphorylation site on glutaminase c
AssigneeCornell University
InventorsKristin Wilson Cerione, Richard A. Cerione
Life of this patent
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Abstract
A method of reducing the production of glutamate from glutamine by glutaminase C in a cell or tissue. The method involves inhibiting activating phosphorylation of glutaminase C under conditions effective to reduce production of glutamate from glutamine. Methods for treating or preventing a condition mediated by the activating phosphorylation of glutaminase C, detecting a condition mediated by the activating phosphorylation of glutaminase C, and screening for compounds capable of treating or preventing cancer are also disclosed.
Claims33
A method of reducing production of glutamate from glutamine in a cell or a tissue, said method comprising:
- inhibiting activating phosphorylation within the amino acid sequence of SEQ ID NO:
- 1 of glutaminase C in the cell or tissue under conditions effective to reduce production of glutamate from glutamine.
A method of treating or preventing a condition mediated by activating phosphorylation of glutaminase C in a subject, said method comprising:
- selecting a subject having or being susceptible to a condition mediated by the activating phosphorylation within the amino acid sequence of SEQ ID NO:
- 1 of glutaminase C and
- administering to said selected subject an inhibitor of the activating phosphorylation within the amino acid sequence of SEQ ID NO:
- 1 of glutaminase C activity under conditions effective to treat or prevent the condition mediated by the activating phosphorylation of glutaminase C.
A method of detecting a condition mediated by activating phosphorylation within the amino acid sequence of SEQ ID NO:
- 1 of glutaminase C, said method comprising:
- providing a cell or tissue;
- providing a reagent that specifically recognizes the activating phosphorylation within the amino acid sequence of SEQ ID NO:
- 1 of glutaminase C;
- contacting the cell or tissue with the reagent under conditions effective for the reagent to bind to phosphorylated glutaminase C and form a phosphorylated glutaminase C-reagent conjugate;
- and
- identifying presence of the phosphorylated glutaminase C-reagent conjugate wherein the formation of the phosphorylated glutaminase C-reagent conjugate indicates the existence of a condition mediated by the activating phosphorylation of glutaminase C.
A method of screening for compounds capable of treating or preventing cancer, said method comprising:
- providing a cancer cell or cancer tissue under conditions effective to produce glutamate from glutamine as a result of glutaminase C activity;
- providing a plurality of candidate compounds;
- contacting the cancer cell or cancer tissue with the candidate compounds under conditions effective for activating phosphorylation;
- and
- identifying the candidate compounds which inhibit the activating phosphorylation of glutaminase C within the amino acid sequence of SEQ ID NO:
- 1 as a result of said contacting as having potential capability of treating or preventing cancer.
Dependencies are inferred from the claim text.