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US 2013/0252852 A1Published application
Oligonucleotides Related to Lipid Membrane Attachment
AssigneeBio-Rad Laboratories, Inc.
InventorsIndriati Pfeiffer, Fredrick Höök
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AssigneeBio-Rad Laboratories, Inc.
InventorsIndriati Pfeiffer, Fredrick Höök
Estimating the term…
A method of forming a lipid membrane attached linker comprises contacting a lipid membrane with an oligonucleotide having a first strand and a second strand of nucleic acid and two or more hydrophobic anchoring moieties located in its terminal ends. The two strands are hybridized to each other in a duplex section in a manner that the first strand terminal end is not a part of the duplex section and free from a hydrophobic anchoring moiety and the two or more hydrophobic anchoring moieties are covalently attached to the adjacent terminal ends of the first strand and the second strand of said oligonucleotide, thereby accomplishing a direct attachment of the oligonucleotide by the moieties on the same membrane.
A method of forming a lipid membrane attached linker, comprising contacting a lipid membrane with an oligonucleotide having a first strand and a second strand of nucleic acid and two or more hydrophobic anchoring moieties located in its terminal ends, said two strands being hybridized to each other in a duplex section in a manner that the first strand terminal end is not a part of said duplex section and free from a hydrophobic anchoring moiety and wherein said two or more hydrophobic anchoring moieties are covalently attached to the adjacent terminal ends of said first and second strands of said oligonucleotide, thereby accomplishing a direct attachment of said oligonucleotide by said moieties on the same membrane.
Dependencies are inferred from the claim text.