Methods for profiling and quantitating cell-free rna
OwnerLian Chye Winston Koh, Stephen R. Quakeinventors
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Abstract
The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected.
Claims30
A method of assessing the health of a tissue, the method comprising
- detecting a sample level of RNA in a biological sample;
- comparing the sample level of RNA to a reference level of RNA specific to the tissue;
- determining whether a difference exists between the sample level and the reference level;
- and
- characterizing the tissue as abnormal if a difference is detected.
A method of assessing health of a tissue, the method comprising characterizing the tissue as abnormal if a specified level of RNA is present in the blood.
A method for detecting differential transcript levels from a biological sample comprising a mixture of genetic material from different genomic sources, the method comprising the steps of:
- a) obtaining a plurality of biological samples over different time-points, each sample containing a mixture of genetic material from different genomic sources;
- b) amplifying a plurality of RNA transcripts from the plurality of biological samples to obtain a plurality of amplified samples, each containing a mixture of amplified RNA transcripts from different genomic sources;
- c) detecting the levels of one or more of the RNA transcripts from each of the amplified samples;
- and
- d) conducting an analysis that compares the levels of one or more of the RNA transcripts between each of the amplified samples to determine a differential profile for one or more of the detected RNA transcripts across the different time-points.
Dependencies are inferred from the claim text.