Compositions and methods for increased ethanol titer from biomass
AssigneeCargill, Incorporated
InventorsHolly J. Jessen, Jian Yi
Life of this patent
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Abstract
The present application discloses the identification of novel I. orientalis ADH1, ADHa, and ADHb genes, and the production and characterization of genetically modified yeast cells in which these genes were altered. Provided herein are isolated I. orientalis ADH1, ADHa, and ADHb polynucleotides and polypeptides, genetically modified yeast cells that overexpress I. orientalis ADH1 and/or contain deletions or disruptions of ADHa and/or ADHb, and methods of using culturing these modified cells to produce ethanol.
Claims29
1-12. (canceled)
A genetically modified yeast cell that overexpresses a polypeptide comprising an amino acid sequence with at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:6.
(canceled)
(canceled)
A genetically modified yeast cell that overexpresses a polypeptide comprising an amino acid sequence with at least 85% sequence identity to the amino acid sequence set forth in SEQ ID NO:6, wherein said polypeptide is capable of catalyzing the conversion of acetaldehyde to ethanol.
(canceled)
A genetically modified yeast cell comprising a deletion or disruption of a gene encoding a polypeptide comprising an amino acid sequence with at least 90% sequence identity to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:2 and SEQ ID NO:4.
21-22. (canceled)
A genetically modified yeast cell comprising a deletion or disruption of a gene encoding a polypeptide comprising an amino acid sequence with at least 85% sequence identity to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:2 and SEQ ID NO:4, wherein said polypeptide is capable of catalyzing the conversion of ethanol to acetaldehyde.
(canceled)
A genetically modified yeast cell that overexpresses a first polypeptide comprising an amino acid sequence with at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:6 and comprises a deletion or disruption of a gene encoding a second polypeptide comprising an amino acid sequence with at least 90% sequence identity to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:2 and SEQ ID NO:4.
27-28. (canceled)
30-31. (canceled)
A genetically modified yeast cell that overexpresses a first polypeptide comprising an amino acid sequence with at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:6 and comprises a deletion or disruption of a gene encoding a second polypeptide comprising an amino acid sequence with at least 70% sequence identity to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NO:2 and SEQ ID NO:4, wherein said first polypeptide is capable of catalyzing the conversion of acetaldehyde to ethanol, and wherein said second polypeptide is capable of catalyzing the conversion of ethanol to acetaldehyde.
41-44. (canceled)
Dependencies are inferred from the claim text.