Loading patent…
AssigneeGevo, Inc.
InventorsReid M. Renny Feldman, Uvini Gunawardena, Jun Urano, Peter Meinhold, Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith
Estimating the term…
The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
-20. (canceled)
A recombinant yeast microorganism for producing an acetolactate intermediate, wherein the recombinant yeast microorganism has been engineered to:
The recombinant yeast microorganism of claim 1, wherein all endogenous PDC genes and all endogenous GPD genes are disrupted, mutated, or deleted.
The recombinant yeast microorganism of claim 1, wherein said one or more endogenous PDC genes is selected from the group consisting of PDC 1, PDC2, PDC5, and PDC6.
The recombinant yeast microorganism of claim 1, wherein said one or more endogenous GPD genes is selected from the group consisting of GPD1 and GPD2.
The recombinant yeast microorganism of claim 1, wherein said ALS is a cytosolically-localized ALS.
The recombinant yeast microorganism of claim 5, wherein said cytosolically-localized ALS is encoded by the Lactococcus lactis alsS gene.
The recombinant yeast microorganism of claim 5, wherein said cytosolically-localized ALS is encoded by the Bacillus subtilis alsS gene.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism has been engineered to disrupt or delete an endogenous pyruvate dehydrogenase (PDH) gene.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism further expresses a heterologous gene encoding a ketol-acid reductoisomerase.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism further expresses a heterologous gene encoding a dihydroxy acid dehydratase.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism further expresses a heterologous gene encoding an α-ketoisovalerate decarboxylase.
The recombinant yeast microorganism of claim 11, wherein said heterologous gene encoding an α-ketoisovalerate decarboxylase is obtained from Lactococcus lactis.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism expresses a heterologous gene encoding an alcohol dehydrogenase.
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism expresses
The recombinant yeast microorganism of claim 1, wherein said recombinant yeast microorganism is a yeast of a genus selected from the group consisting of Saccharomyces, Kluyveromyces, Candida, Pichia, Hansenula, and Schizosaccharomyces.
The recombinant yeast microorganism of claim 14, wherein said recombinant yeast microorganism is a yeast of a genus selected from the group consisting of Saccharomyces, Kluyveromyces, Candida, Pichia, Hansenula, and Schizosaccharomyces.
A method of producing isobutanol, comprising:
Dependencies are inferred from the claim text.