Novel cellulase derived from thermosporothrix hazakensis

A method for saccharification of a carbohydrate-containing raw material comprising treating a carbohydrate-containing raw material with a cellulase derived from Thermosporothrix hazakensis having enzyme activity on at least β-glucan, soluble cellulose, crystalline cellulose, phosphoric acid-swollen cellulose, and xylan, a polynucleotide encoding the cellulose, an expression vector comprising the polynucleotide, a transformant obtained with the use of the expression vector or a culture product obtained by culturing the transformant.

A method for producing a food or feed product comprising treating a carbohydrate-containing raw material with a cellulase derived from Thermosporothrix hazakensis having enzyme activity on at least β-glucan, soluble cellulose, crystalline cellulose, phosphoric acid-swollen cellulose, and xylan, a polynucleotide encoding the cellulose, an expression vector comprising the polynucleotide, a transformant obtained with the use of the expression vector or a culture product obtained by culturing the transformant.

A method for producing ethanol comprising:

• (i) treating a carbohydrate-containing raw material with a cellulase derived from Thermosporothrix hazakensis having enzyme activity on at least β-glucan, soluble cellulose, crystalline cellulose, phosphoric acid-swollen cellulose, and xylan, a polynucleotide encoding the cellulose, an expression vector comprising the polynucleotide, a transformant obtained with the use of the expression vector or a culture product obtained by culturing the transformant;
• and
• (ii) subjecting the product obtained in step (i) to fermentation.