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US 2013/0252249 A1Published application
Method for Separating and Detecting an Analyte
AssigneeRoche Molecular Systems, Inc.
InventorsRenato Belz, Andreas Gisler, Robert Huesler, Rolf Knobel, Christian Thalmann
Life of this patent
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Abstract
A method for separating and analyzing an analyte is provided which comprises, in a first position, transferring a liquid sample to a multiwell plate with a pipette tip, replacing the tips in the tip rack in the same position, and re-using the pipette tips for aspirating and dispensing liquid.
Claims14
A method for isolating and analyzing an analyte that may be present in a fluid sample, the method comprising the automated steps of:
- a) transferring said fluid sample from a sample vessel to a processing vessel with a pipette tip of a first type;
- b) combining together a solid support material and said fluid sample in a well of said processing vessel for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
- c) isolating the solid support material from other material present in the fluid sample in a separation station;
- and
- d) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the materials one or more times with a wash buffer;
- wherein said pipette tip of a first type used in step a) is re-used after step a), and wherein said pipette tip of a first type is stored in a rack comprising pipette tips of a first type and pipette tips of a second type.
3 more elements
An analytical system for processing an analyte, said system comprising:
- a) a first position comprising a first receptacle holding a liquid sample comprising an analyte, a second receptacle for holding a liquid sample, a rack holding pipette tips, and a first process head for transferring a liquid sample from the first receptacle to a second receptacle;
- b) a second position comprising a station for receiving said second receptacle, and a rack holding station for receiving said rack;
- and
- c) a transfer system for transferring the second receptacle and the rack holding pipette tips between the first position and the second position.
1 more element
An automated analyzer for detecting an analyte comprising
- a) a plurality of stations disposed within said analyzer, wherein the plurality of stations comprises:
- i) a sample dispensing station disposed in a first location, said sample dispensing station being constructed and arranged to dispense liquid sample comprising an analyte from a sample vessel to a processing vessel with pipette tips held in a rack;
- ii) a separation station disposed in a second location, wherein said separation station is constructed and arranged to receive said processing vessel holding said liquid sample and said rack holding pipette tips used in the sample dispensing station and to separate an analyte from other material present in the liquid sample;
- and
- iii) a reaction station disposed in a third location, wherein said reaction station comprises a temperature-controlled incubator for incubating said analyte with reagents necessary to obtain a detectable signal;
- b) a transport mechanism constructed and arranged to transport said sample vessel and said rack from said sample dispensing station to said separation station.
3 more elements
Dependencies are inferred from the claim text.