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US 2013/0252243 A1Published application
Methods of determining potency of chemically-synthesized oligonucleotides
AssigneeIsis Pharmaceuticals, Inc.
InventorsWalter F. Lima, Timothy Vickers, Stanley T. Crooke
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Abstract
Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.
Claims13
A method of determining relative potency of a chemically-synthesized RNAi agent comprising:
- forming a first test sample comprising the chemically-synthesized RNAi agent and Ago2;
- forming a second test sample comprising an RNAi control agent and Ago2;
- and
- measuring the Ago2 activity of the chemically-synthesized RNAi agent and the Ago2 activity of the RNAi control agent, wherein the difference in the Ago2 activity of the chemically-synthesized RNAi agent and Ago2 activity of the control RNAi agent indicates potency of the chemically-synthesized RNAi agent relative to the control RNAi agent.
1 more element
Dependencies are inferred from the claim text.