Method and device for separating charged particles in liquid sample and manufacturing method of the device

A device for separating charged particles in a liquid sample, comprising:

• a substrate layer;
• an interlaid layer disposed on the substrate layer;
• a cover layer disposed on the interlaid layer, wherein the substrate layer, the interlaid layer and the cover layer together define a channel;
• and
• two electrodes correspondingly disposed on the cover layer and the substrate layer, respectively, wherein the electrodes do not contact with the liquid sample.

.A manufacturing method of a device for separating charged particles in a liquid sample, wherein the device comprises a substrate layer, an interlaid layer, a cover layer and two electrodes, the manufacturing method comprising the steps of:

• forming an opening and an outlet on the cover layer;
• forming two electrodes on the cover layer and the substrate layer, respectively;
• disposing an optical paste or a photoresist material on the substrate layer to form the interlaid layer;
• processing the interlaid layer;
• and
• combining the cover layer, the interlaid layer and the substrate layer to together define a channel, wherein the channel does not contact with the electrodes;
• wherein, the interlaid layer is disposed on the substrate layer, the cover layer is disposed on the interlaid layer, and the electrodes do not contact with the liquid sample.

A manufacturing method of a device for separating charged particles in a liquid sample, wherein the device comprises a substrate layer, an interlaid layer, a cover layer and two electrodes, the manufacturing method comprising the steps of:

• forming an opening and an outlet on the cover layer;
• forming two electrodes on the cover layer and the substrate layer, respectively;
• disposing an optical paste or a photoresist material on the substrate layer to form the interlaid layer;
• processing the interlaid layer;
• and
• combining the cover layer, the interlaid layer and the substrate layer to together define a channel, wherein the channel does not contact with the electrodes;
• wherein, the interlaid layer is disposed on the substrate layer, the cover layer is disposed on the interlaid layer, the electrodes do not contact with the liquid sample, and the sides of the electrodes opposite to each other have different surface areas so as to form a non-uniform electric field.