Back to results
US 2013/0252227 A1Published application
Apparatus and Method for Providing Cryopreserved ECP-Treated Mononuclear Cells
AssigneeFenwal, Inc.
InventorsKyungyoon Min, Katherine Radwanski, Cheryl Heber
Life of this patent
Estimating the term…
Abstract
An apparatus and method for providing cryopreserved mononuclear cells that have been treated by extracorporeal photopheresis (“ECP”) is disclosed. More specifically, the present disclosure relates to providing ECP treated mononuclear cells that retain their apoptotic properties after cryopreservation and subsequent thawing.
Claims20
A method of providing a cryopreserved treated mononuclear cell product comprising:
- combining a mononuclear cell product with a photoactive compound that is activated by exposure to light of a selected wavelength,
- exposing the mononuclear cell product combined with said photoactive compound to light for a selected period of time at the selected wavelength to obtain a treated mononuclear cell product,
- combining at least a portion of the treated mononuclear cell product with a cryopreservation medium,
- cryopreserving at least said portion of said treated mononuclear cell product.
1 more element
A cyopreserved treated mononuclear cell product comprising:
- about 50 mL to about 300 mL of a mononuclear cell product that has been exposed to a selected dose of ultraviolet light;
- and
- about 50 mL to about 300 mL of a cryopreservation solution.
An apparatus for providing mononuclear cells that have been treated by extracorporeal photopheresis comprising:
- (a) a disposable fluid circuit comprising:
- i. a processing chamber for separating whole blood into one or more components including mononuclear cells,
- ii. at least one auxiliary storage container,
- (b) a separation device adapted to receive said processing chamber for effecting separation of said mononuclear cells from whole blood,
- (c) a programmable controller programmed to:
- i. separate mononuclear cells from said whole blood in said processing chamber,
- ii. irradiate said mononuclear cells with ultraviolet light to produce treated mononuclear cells,
- iii. convey at least a portion of said treated mononuclear cells into said auxiliary storage container for cryopreservation of said treated cells within said auxiliary container.
5 more elements
Dependencies are inferred from the claim text.