Method for screening compounds for treating sepsis targeting nod2 signalling pathway and composition for treating sepsis comprising nod2 signalling pathway inhibitors
AssigneeSnu R&Db Foundation
InventorsDoo Hyun CHUNG, Sae Jin OH, Ji Hyung KIM
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Abstract
Methods for screening compounds for treating sepsis are disclosed. The present methods and compositions are targeting NOD2 mediated signaling pathway and the agents identified by the present methods are qualified as drug candidates for clinical development. Further Methods and composition for treating sepsis are disclosed.
Claims16
A method of screening a therapeutic agent for sepsis, which comprises:
- (i) providing a RIP2 protein;
- (ii) contacting the RIP2 protein with a test substance wherein the substance is expected to inhibit the phosphorylation or kinase activity of the RIP2 protein;
- and
- (iii) selecting a test substance as a candidate that decreases the phosphorylation level or the kinase activity of the RICK protein in comparison to a negative control that is not contacted with a test substance.
A method of screening a therapeutic agent for sepsis comprising:
- (i) providing either a NOD2 or the CARD region of a NOD2 protein, and either a RIP2 or the CARD region of a RIP2 protein;
- (ii) contacting the proteins with a test substance wherein the substance is expected to interfere with the interaction of the NOD2 or the CARD region of the NOD2 with the RIP2 or the CARD region of the RIP2 protein;
- and
- (iii) detecting the interaction of (ii) and selecting a test substance as a candidate that interfere with the interaction.
A method of treating sepsis comprising administering to a subject in need thereof an effective amount of an inhibitor of the kinase activity of a RIP2 protein and/or an inhibitor of the phosphorylation of a RIP2 protein.
A method of treating sepsis comprising administering to a subject in need thereof an effective amount of one or more NOD2 inhibitors.
A method of treating sepsis comprising administering to a subject in need thereof an effective amount of one or more RIP2 inhibitors.
A method of treating sepsis comprising administering to a subject in need thereof an effective amount of one or more inhibitors that interfere with RIP2 and NOD2 interaction.
Dependencies are inferred from the claim text.