Methods of isolating and culturing stem cells
AssigneeTissuetech, Inc.
InventorsScheffer Tseng, Szu-Yu Chen, Suzhen Zhang
Life of this patent
Estimating the term…
Abstract
Provided herein are methods of isolating and expanding a plurality of multipotent stem cells. Also described are methods of expanding stem cells on a substrate comprising an HC-HA complex. Also described are isolated and expanded stem cells produced by the methods and uses thereof, including stem cell therapy, as niche cells for supporting other types of stem cells, or as bioreactors for the production of HC-HA complexes. Also described are uses of HC-HA complexes as a carrier for stem cells.
Claims155
A method for isolating an E-cadherin positive stem cell, comprising contacting a mixed cell population comprising one or more stem cells with an agent that binds to E-cadherin, thereby isolating an E-cadherin positive stem cell.
A method for expanding a stem cell population comprising:
- expanding one or more stem cells in a culture comprising a two-dimensional substrate comprising an HC-HA complex, thereby forming a plurality of expanding stem cells.
A method for expanding a stem cell comprising:
- (a) separating a plurality of cells of a tissue sample from components of an extracellular matrix in the tissue sample, to form a mixed cell population;
- (b) culturing the mixed cell population in a first culture comprising supplemented hormonal epithelial medium (SHEM) on a plastic tissue culture dish, thereby producing a population of non-adherent cells;
- (c) isolating the population of non-adherent cells;
- and
- (d) expanding at least one cell of the population of non-adherent cells in a second culture comprising a two-dimensional substrate, thereby forming a plurality of expanding stem cells.
A method for inducing or maintaining pluripotency in a cell, comprising culturing the cell with an HC-HA complex, thereby inducing or maintaining pluripotency in the cell.
A method for producing an isolated native HC-HA complex comprising:
- (a) culturing an amniotic membrane stem cell, thereby producing a cultured amniotic membrane stem cell;
- and
- (b) isolating an HC-HA complex from the cultured stem cell, thereby producing an isolated native HC-HA complex.
A composition comprising:
- (a) a stem cell;
- (b) an HC-HA complex;
- and
- (c) a tissue culture plate.
Dependencies are inferred from the claim text.