Ulvan lyase, method for manufacturing same, and uses thereof

A method of manufacturing an ulvan lyase, the method comprising:

• genetic recombination using a nucleic acid or a vector to produce the ulvan lyase, the nucleic acid chosen from SEQ ID No. 5, SEQ ID No. 5 with sequence SEQ ID No. 6 at its 3′ end, SEQ ID No. 5 with sequence SEQ ID No. 7 at its 5′ end, or SEQ ID No. 5 with sequence SEQ ID No. 6 at its 3′ end and sequence SEQ ID No. 7 at its 5′ end, and the vector comprising any of the foregoing nucleic acids;
• wherein the ulvan lyase of 30 or 46 kD comprising at least one of the following four sequences in its peptide sequence:
• PNDPNLK,
• LLEVGNIGTFGSTGS,
• DLANPDNV,
• and
• WNLPE.

A method of degrading ulvans comprising a step of

• bringing the ulvans into contact with an ulvan lyase, a host cell, or a microorganism in conditions permitting degradation of the ulvans by enzymatic digestion by said protein or said host or said microorganism;
• wherein the ulvan lyase of 30 or 46 kD comprising at least one of the following four sequences in its peptide sequence:
• PNDPNLK,
• LLEVGNTGTFGSTGS,
• DLANPDNV,
• and
• WNLPE;
• and
• wherein the host cell comprises a nucleic acid chosen from SEQ ID No. 5, SEQ ID No. 5 with sequence SEQ ID No. 6 at its 3′ end, SEQ ID No. 5 with sequence SEQ ID No. 7 at its 5′ end, or SEQ ID No. 5 with sequence SEQ ID No. 6 at its 3′ end and sequence SEQ ID No. 7 at its 5′ end;
• and
• wherein the microorganism is a culture of the microorganism deposited on 17 Jun. 2010 under number I-4324 in the National Collection of Cultures of Microorganisms (CNCM) 25 rue du docteur Roux, 75724 Paris Cedex 15, France.