US 2013/0200277 A1 · SYSTEM AND METHOD FOR DETECTING MULTIPLE-EXCITATION-INDUCED LIGHT IN A FLOW CHANNEL

Abstract

A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

Claims18

Dependent claims

A system for detecting signal components of light induced by multiple excitation sources, comprising:

a flow channel configured for the flow of particles, the flow channel comprising at least two spatially separated optical interrogation zones;
a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones;
a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones, wherein the second wavelength is different from the first wavelength;
a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal;
and
a processor configured to receive the total electrical signal from the detector subsystem, to de-modulate electrical signal that is modulated, and to determine signal components from the light detected from each of the optical interrogation zones.

3 more elements

A method of detecting signal components from light induced by multiple excitation sources, the method comprising:

providing a flow channel comprising at least two spatially separated optical interrogation zones;
flowing a population of particles labeled with at least two different fluorescent molecules through each of the optical interrogation zones;
directing a light beam of a first wavelength at a near constant intensity at a first of the optical interrogation zones to induce emission of light from the fluorescence-molecule containing particles;
directing a light beam of a first wavelength with an intensity modulated over time according to a modulating frequency onto a second of the optical interrogation zones to induce emission of light from the fluorescence-molecule containing particles, wherein the second wavelength is different from the first wavelength;
detecting the light emitted from the particles from each of the optical interrogation zone and converting detected light into a total electrical signal;
de-modulating electrical signal from the total electrical signal;
and
determining signal components of the light detected from each of the optical interrogation zones.

5 more elements

Dependencies are inferred from the claim text.

System and method for detecting multiple-excitation-induced light in a flow channel

Life of this patent

System and method for detecting multiple-excitation-induced light in a flow channel

Life of this patent

Abstract

Claims18