Chromatography Methods
AssigneeIronstone Separations, Inc.
InventorsJames D. McChesney, Douglas L. Rodenburg
Life of this patent
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Abstract
This application discloses, in part, 1) a stationary phase column and compression designs for preparative chromatography, 2) a method of improving performance of silica gel chromatography by controlling the hydration of silica gel and acidifying the mobile phase, and 3) a method of extending the life of a silica gel column packing by cleaning or regenerating the silica gel stationary phase.
Claims16
A method for improving a separation variable in a normal phase column chromatographic separation method for the separation of a composition comprising a mixture of components, wherein the method comprises increasing the water content in a mobile phase that is used as an eluting solvent in the normal phase column chromatographic separation method.
A method for regenerating a previously processed packing material in a column of a column chromatographic separation apparatus for the separation of a composition comprising a mixture of components, wherein the packing material having an original equilibrium water content has been used in a chromatographic cycle for the separation of the composition using an organic mobile phase solvent, the method comprising an addition of a polar solvent having a higher dielectric constant than that of the mobile phase solvent, to the organic mobile phase solvent to form a regeneration solvent, cycling the regeneration solvent through the previously processed packing material, followed by cycling the organic mobile phase solvent through the packing material to regenerate the packing material substantially to its original equilibrium water content.
A chromatographic solvent composition for the chromatographic separation of a composition comprising a mixture of components using a silica gel packing material in a chromatographic column, wherein the chromatographic solvent composition has an equilibrium water content comparable to that of an original equilibrium water content of the packing material, the chromatographic solvent composition comprises a water content in the mobile phase of about 15%, 10%, 5%, 4%, 3%, 2%, 1% wt/wt or less.
A preparative chromatography column comprising a column tube having a proximal end and a distal end, a proximal distribution assembly comprising an inlet feature on an inlet piston in the proximal end of the column tube, a distal distribution assembly comprising an outlet feature on an outlet piston in the distal end of the column tube, a bed volume between the inlet piston and the outlet piston, wherein the inlet piston is coupled with a thrust washer, a disc spring washer, a disc spring washer guide and a compression cylinder, and wherein the inlet piston is biased against the disc spring for compressing the disc spring washer when the bed volume is filled with a stationary phase.
Dependencies are inferred from the claim text.