Cell-based production of nanulosonates
AssigneesUniversity of Ottawa, National Research Council of Canada
InventorsChristopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
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Abstract
The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose. Methods for producing the sugars are also provided.
Claims40
A recombinant cell for the production of pseudaminic acid, comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding PseB enzyme function, a gene encoding PseC enzyme function, a gene encoding PseH enzyme function, a gene encoding PseI enzyme function, a gene encoding PseG enzyme function, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
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The method of claim 20, wherein growth medium for the recombinant cell is supplemented with palmitate.
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A recombinant cell for the production of legionaminic acid, comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding PglF enzyme function, a gene encoding PglE enzyme function, a gene encoding PglD enzyme function, a gene encoding LegI enzyme function, a gene encoding LegG enzyme function, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
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The method of claim 21, wherein growth medium for the recombinant cell is supplemented with palmitate.
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A recombinant cell for the production of UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose (UDP-6-deoxy-AltdiNAc), comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding PseB enzyme function, a gene encoding PseC enzyme function, a gene encoding PseH enzyme function, a gene encoding GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
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A recombinant cell for the production of UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose (UDP-BacdiNAc), comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding PglF enzyme function, a gene encoding PglE enzyme function, a gene encoding PglD enzyme function, a gene encoding GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
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Dependencies are inferred from the claim text.