Use of non-agrobacterium bacterial species for plant transformation
OwnerXudong Ye, JunJiang Shen +1inventors
InventorsXudong Ye, JunJiang Shen, Edward Williams
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Abstract
The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
Claims36
(canceled)
A method for transforming a plant cell, comprising:
- (a) contacting at least a first plant cell with a bacterium other than Agrobacterium comprising (i) a first nucleic acid required for conjugative transfer of DNA sequences independent of VirD2 function, and (ii) a second nucleic acid comprising a nucleic acid of interest;
- wherein the plant cell is a soybean, canola, corn, or cotton plant cell and wherein polypeptides encoded by the nucleic acid required for conjugative transfer act to transfer the nucleic acid of interest into the plant cell;
- and
- (b) selecting at least a first plant cell transformed with the nucleic acid of interest.
28-32. (canceled)
A DNA construct competent for virD2-independent transfer from Rhizobia and lacking T-DNA border sequence, the construct comprising an oriT sequence operably linked to a nucleic acid of interest.
Dependencies are inferred from the claim text.