Method for down-regulating gene expression in fungi
AssigneeDevgen Private Limited
InventorsSimone Dorfmueller, Phuay-Yee Goh, Pascale Feldmann
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Abstract
The present invention concerns methods for controlling and/or preventing fungus infestation on a cell, organism, substrate or material via dsRNA mediated gene silencing. The methods of the invention are particularly used to alleviate pathogenic fungal infestation on plants, plant materials or seeds. Suitable fungal target genes and fragments thereof, expression cassettes, dsRNA molecules, host cells expressing the dsRNA, compositions and transgenic plants and plant cells are provided.
Claims28
A method for preventing and/or controlling pathogenic Rhizoctonia spp. infestation, comprising exposing a pathogenic Rhizoctonia to at least one dsRNA comprising annealed complementary strands, whereby ingestion of said dsRNA by said Rhizoctonia inhibits the expression of a Rhizoctonia target gene, which target gene comprises a nucleotide sequence that is complementary to one of the strands of said dsRNA.
A nucleic acid molecule selected from the group consisting of:
- (i) a nucleic acid molecule which comprises at least 21, preferably at least 22, 23 or 24 contiguous nucleotides of a nucleotide sequence as represented by any of SEQ ID NOs 46, 48, 50, 52, 1, 3, 5, 7, 21, 22, 23, 25, 77, 78, 79, 81, 98, 100, 102, 104, 136, 138, 140, 142, 144, 146, 148, 179, 181, 183, 207, 209, 211, 213, 215, 217 or 250, or the complement thereof, or
- (ii) a nucleic acid molecule which comprises at least 21, preferably at least 22, 23 or 24 contiguous nucleotides of a nucleotide sequence that, when the two sequences are optimally aligned and compared using the BLASTN alignment tool, is at least 75% identical to any of SEQ ID NOs 46, 48, 50, 52, 1, 3, 5, 7, 21, 22, 23, 25, 77, 78, 79, 81, 98, 100, 102, 104, 136, 138, 140, 142, 144, 146, 148, 179, 181, 183, 207, 209, 211, 213, 215, 217 or 250, or the complement thereof, or
- (iii) a nucleic acid molecule encoding an amino acid sequence that, when the two sequences are optimally aligned and compared using the BLASTP alignment tool, is at least 70% identical to any of SEQ ID NOs 47, 49, 51, 2, 4, 6, 24, 80, 99, 101, 103, 137, 139, 141, 143, 145, 147, 180, 182, 208, 210, 212, 214 or 216.
Dependencies are inferred from the claim text.