Methods to Identify Soybean Aphid Resistant Quantitative Trait Loci in Soybean and Compositions Thereof
AssigneesUniversity of Georgia Research Foundation, Inc., Monsanto Technology LLC
InventorsVERGEL CONCIBIDO, JAMES NARVEL, JENNIFER YATES, HENRY ROGER BOERMA
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Abstract
The present invention is in the field of plant breeding and aphid resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trait loci that are associated with resistance to aphids, Aphis glycines. The invention further includes method for monitoring the introgression quantitative trait loci (QTL) conferring aphid resistance into elite germplasm in a breeding program.
Claims25
A method of introgressing an allele into a soybean plant comprising:
- A) Providing a population of soybean plants,
- B) Genotyping at least one soybean plant in the population with respect to a soybean genomic nucleic acid marker selected from the group comprising SEQ ID NO:
- 85 through SEQ ID NO:
- 120,
- C) Selecting from the population at least one soybean plant comprising at least one allele associated with aphid resistance.
A method of introgressing an allele into a soybean plant comprising:
- C) crossing at least one aphid resistant soybean plant with at least one aphid sensitive soybean plant in order to form a segregating population,
- B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains a aphid resistant allele, wherein said aphid resistant allele is an allele selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, and 28 aphid resistant loci where one or more alleles at one or more of their loci are selected from the group consisting of aphid resistance allele 1, aphid resistance allele 2, aphid resistance allele 3, aphid resistance allele 4, aphid resistance allele 5, aphid resistance allele 6, aphid resistance allele 7, aphid resistance allele 8, aphid resistance allele 9, aphid resistance allele 10, aphid resistance allele 11, aphid resistance allele 12, aphid resistance allele 13, aphid resistance allele 14, aphid resistance allele 15, aphid resistance allele 16, aphid resistance allele 17, aphid resistance allele 18, aphid resistance allele 19, aphid resistance allele 20, aphid resistance allele 21, aphid resistance allele 22, aphid resistance allele 23, aphid resistance allele 24, aphid resistance allele 25, aphid resistance allele 26, aphid resistance allele 27, aphid resistance allele 28, aphid resistance allele 29, aphid resistance allele 30, aphid resistance allele 31, aphid resistance allele 32, aphid resistance allele 33, aphid resistance allele 34, aphid resistance allele 35, and aphid resistance allele 36.
A substantially purified nucleic acid molecule for the detection of loci related to aphid resistance comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 1 through SEQ ID NO:
- 175 and complements thereof.
A method of identifying an aphid resistance allele in a soybean plant comprising detecting a locus associated with a SNP marker selected from the group consisting of SEQ ID NO:
- 85 through SEQ ID NO:
- 120.
A set of oligonucleotides comprising:
- A) a pair of oligonucleotide primers wherein each of said primers comprises at least 12 contiguous nucleotides and wherein said pair of primers permit PCR amplification of a DNA segment comprising a soybean genomic DNA polymorphism associated with aphid resistance loci identified in Table 6 and 7;
- and
- B) at least one detector oligonucleotide that permits detection of a polymorphism in said amplified segment, wherein the sequence of said detector oligonucleotide is at least 95 percent identical to a sequence of the same number of consecutive nucleotides in either strand of a segment of maize DNA that include or are immediately adjacent to said polymorphism of step (a).
Dependencies are inferred from the claim text.