Back to results
US 2013/0160153 A1Published application
Humanized Light Chain Mice
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn Macdonald, Cagan Gurer, Karolina A. Hosiawa, Sean Stevens, Andrew J. Murphy
Life of this patent
Estimating the term…
Abstract
Non-human animals, tissues, cells, and genetic material are provided that comprise a modification of an endogenous non-human heavy chain immunoglobulin sequence and that comprise an ADAM6 activity functional in a mouse, wherein the non-human animals express a human immunoglobulin heavy chain variable domain and a cognate human immunoglobulin λ light chain variable domain.
Claims33
A non-human animal comprising
- (a) an insertion of one or more human Vλ and Jλ gene segments upstream of an non-human immunoglobulin light chain constant region,
- (b) an insertion of one or more human VH, one or more human DH and one or more human JH gene segments upstream of an non-human immunoglobulin heavy chain constant region, and
- (c) a nucleotide sequence that encodes an ADAM6 protein or a functional fragment thereof, wherein the ADAM6 protein is expressed from an ectopic ADAM6 nucleic acid sequence.
A genetically modified non-human animal that comprises
- (a) one or more unrearranged human Vλ gene segments and one or more unrearranged human Jλ gene segments at an endogenous immunoglobulin light chain locus of the non-human animal,
- (b) on or more unrearranged human VH gene segments, one or more unrearranged human DH gene segments, and one or more unrearranged human JH gene segments at an endogenous immunoglobulin heavy chain locus of the non-human animal, wherein the non-human animal is capable of expressing an ADAM6 protein or functional fragment thereof.
Dependencies are inferred from the claim text.