Transgenic animal as a model for identifying adult stem cells, and uses thereof
AssigneeUniversite Pierre Et Marie Curie (Paris 6)
InventorsDavid Sassoon, Vanessa Besson, Giovanna Marazzi
Life of this patent
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Abstract
The present invention relates to the use of a transgenic non-human animal, such as a mouse, expressing a reporter gene detectable by a chromogenic, luminescent or fluorescent signal which identifies the cells that express Pw1, or of Pw1-expressing cells or tissues isolated therefrom, as a model for screening a candidate substance for its ability to stimulate adult stem cells, or for monitoring cell aging.
Claims35
-15. (canceled)
An in vitro method for screening a candidate substance for its ability to stimulate adult stem cells, which method comprises the steps consisting of:
- a. providing adult stem cells isolated from a transgenic non-human animal expressing a reporter gene detectable by a chromogenic, luminescent, or fluorescent signal which identifies the cells that express Pw1 that is a marker of adult stem cells;
- b. contacting said stem cells with the candidate substance;
- and
- c. determining the ability of the candidate substance to trigger, maintain or enhance the chromogenic, luminescent or fluorescent signal, whereby identifying the substance as being able to stimulate adult stem cells.
An in vivo method for screening a candidate substance for its ability to stimulate adult stem cells, which method comprises administering a transgenic non-human animal expressing a reporter gene detectable by a chromogenic, luminescent or fluorescent signal which identifies the cells that express Pw1, that is a marker of adult stem cells, with the candidate substance;
- and determining the ability of the candidate substance to trigger, maintain or enhance the chromogenic, luminescent or fluorescent signal, whereby identifying the substance as being able to stimulate adult stem cells.
A method for cultivating non-human animal adult stem cells, which method comprises the steps consisting of:
- a. providing a transgenic non-human animal expressing a reporter gene detectable by a chromogenic, luminescent or fluorescent signal which identifies the cells that express Pw1 that is a marker of adult stem cells;
- b. isolating adult stem cells from the transgenic non-human animal by analyzing the chromogenic, luminescent or fluorescent signal of the reporter gene, optionally coupled to an analysis of stem cell marker(s);
- and
- c. allowing the isolated adult stem cells to grow in vitro,
A method for screening a candidate substance for its ability to stimulate adult stem cells, which method uses a transgenic non-human animal model expressing a reporter gene detectable by a chromogenic, luminescent or fluorescent signal which identifies the cells that express Pw1, or of Pw1 -expressing cells or tissues isolated therefrom.
A method for monitoring cell aging, which method uses a transgenic non-human animal model expressing a reporter gene detectable by a chromogenic, luminescent or fluorescent signal which identifies the cells that express Pw1, or of Pw1 -expressing cells or tissues isolated therefrom.
Dependencies are inferred from the claim text.