System for Glycated Protein Detection
AssigneeVeritide Limited
InventorLou Reinisch
Life of this patent
Estimating the term…
Abstract
A method of detecting the presence of glycated proteins or peptides (GPs) in a sample comprises carrying out the steps of assessing the sample for fluorescence, subjecting the sample to UV radiation, and reassessing the sample for an increase in fluorescence relative to any fluorescence assessed in said first assessing step, an increase in fluorescence at said reassessing step being indicative of the presence of GPs. The method may be useful for detecting disease such as diabetes.
Claims32
A method of detecting the presence of glycated proteins or peptides in a sample comprising carrying out the steps of assessing the sample for fluorescence, subjecting the sample to UV radiation, and reassessing the sample for an increase in fluorescence relative to any fluorescence assessed in said first assessing step, the presence of fluorescence at said assessing step and an increase in fluorescence at said reassessing step being indicative of the presence of glycated proteins or peptides.
A detector for detecting for the presence of glycated proteins or peptides in a sample comprising a UV source, a detection zone within which the sample may be placed or may pass, means for fluorescence analysis arranged to assess for the presence of glycated proteins or peptides by assessing the sample for fluorescence, subjecting the sample to UV radiation, and then reassessing the sample for an increase in fluorescence relative to any fluorescence assessed in said first assessing step, the presence of fluorescence at said assessing step and an increase in fluorescence at said reassessing step being indicative of the presence of glycated proteins or peptides.
Apparatus for detecting glycated proteins or peptides in a sample comprising:
- a source or sources of UV radiation disposed for irradiating a sample and operable to irradiate the sample with UV radiation having a wavelength effective to cause fluorescence of the sample and subsequently with UV radiation having a wavelength effective to cause a photochemical change in the sample;
- a radiation detector disposed for receiving fluorescence radiation from the sample when irradiated by said source or sources of UV radiation and generating a fluorescence signal upon receiving said fluorescence radiation;
- and
- a computer apparatus operatively connected to said radiation detector or detectors and programmed to (a) record a first fluorescence signal from said radiation detector after the sample is first irradiated with UV radiation having a wavelength effective to cause fluorescence of the sample;
- (b) record a second fluorescence signal from said radiation detector after the sample is irradiated with UV radiation having a wavelength effective to cause a photochemical change in the sample and then re-irradiated with UV radiation having a wavelength effective to cause fluorescence of the sample;
- (c) compare said first and second fluorescence signals;
- and then (d) determine if there is any enhancement of the fluorescence of the sample to thereby detect the presence of glycated proteins or peptides.
A method of diagnosing diabetes in a person or a likelihood of a person becoming diabetic, which comprises assessing the skin or a blood sample of the person for fluorescence, subjecting the skin or blood sample to UV radiation, and reassessing the skin or blood sample for an increase in fluorescence relative to any fluorescence assessed in said first assessing step, the presence of fluorescence at said assessing step and an increase in fluorescence at said reassessing step being indicative of the presence of diabetes or likelihood of the person becoming diabetic.
Dependencies are inferred from the claim text.