Method for isolating and/or purifying nucleic acid(s)
AssigneeQiagen GmbH
InventorsRoland Fabis, Jan Petzel
Life of this patent
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Abstract
The present invention relates to a method for isolating and/or purifying one or more nucleic acid(s) from a sample, comprising the steps of essentially separating the nucleic acid(s) from the sample by binding the nucleic acid(s) to a solid phase by means of a non-chaotropic water-soluble binding ligand at a first pH (pH I), and essentially eluting the nucleic acid(s) from the solid phase at a second pH (pH II). The invention further relates to a kit for isolating and/or purifying nucleic acid(s) from a sample and/or for protecting nucleic acid(s).
Claims52
-15. (canceled)
A method for isolating and/or purifying one or more nucleic acid(s) from a sample, comprising:
- (1) separating the nucleic acid(s) from the sample by binding the nucleic acid(s) to a solid phase by means of a binding ligand at a first pH (pH I), and
- (2) eluting the nucleic acid(s) from the solid phase at a second pH (pH II),
- wherein the binding ligand is a non-chaotropic water-soluble cationic compound comprising at least one basic moiety and/or at least one quaternary ammonium moiety, said binding ligand forms a complex with the nucleic acid(s) at a pH below or equal to pH I and wherein the solid phase and the binding ligand are brought into contact upon or after contacting the nucleic acid(s) with the binding ligand, but not before.
A kit for isolating and/or purifying nucleic acid(s) from a sample and/or for protecting nucleic acids, comprising
- (a) a binding ligand, and
- (b) preferably at least one further component selected from the group consisting of:
- (i) a solid phase,
- (ii) a binding buffer,
- (iii) an elution buffer, and
- (iv) instructions for using the kit.
Dependencies are inferred from the claim text.