Process for conjugation of nhs esters with oligonucleotides
AssigneeSigma-Aldrich Co.
InventorRobert William Ball
Life of this patent
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Abstract
The present invention provides processes for the conjugation of NHS esters to amino-modified oligonucleotides. The processes provide the amino-modified oligonucleotide on a solid support such that conjugation can be carried out under conditions that can accommodate a wide variety of NHS esters and oligonucleotides.
Claims20
A process for conjugating a N-hydroxysuccinimide ester with an amino-modified oligonucleotide, the process comprising contacting the N-hydroxysuccinimide ester with the amino-modified oligonucleotide, wherein the amino-modified oligonucleotide is non-covalently bound to an ion exchange support.
A process for conjugating an N-hydroxysuccinimide ester with an amino-modified oligonucleotide, the process comprising contacting the N-hydroxysuccinimide ester with the amino-modified oligonucleotide bound to a solid support, wherein the process is conducted in a non-aqueous environment.
A process for producing an oligonucleotide comprising an R1 moiety, the process comprising:
- (a) contacting an amino-modified oligonucleotide with an ion exchange support such that the amino-modified oligonucleotide binds to the support;
- (b) contacting the supported amino-modified oligonucleotide with an N-hydroxysuccinimide ester comprising R1;
- and
- (c) eluting the oligonucleotide comprising R1,
- wherein R1 is chosen from hydrocarbyl and substituted hydrocarbyl.
Dependencies are inferred from the claim text.