Alkenyl Substituted Cycloaliphatic Compounds as Chemical Inducers of Proximity
AssigneeThe Board of Trustees of the Leland Standford Junior University
InventorsFu-Sen Liang, Gerald R. Crabtree
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Abstract
Methods of inducing proximity of chimeric molecules in a cell are provided. Aspects of the methods include contacting a cell with an amount of alkenyl substituted cycloaliphatic (ASC) inducer compound, e.g., abscisic acid, effective to induce proximity of first and second chimeric molecules. Also provided are compositions and kits for practicing various embodiments of the methods. Methods of the invention find use in a variety of different applications, including transcription induction applications.
Claims18
A method of inducing proximity of first and second chimeric molecules in a cell, the method comprising:
- contacting the cell with an amount of an alkenyl substituted cycloaliphatic (ASC) inducer compound effective to induce proximity of the first and second chimeric molecules, wherein the first and second chimeric molecules each comprise an ASC inducer domain and an effector domain.
A nucleic acid comprising a coding sequence for a chimeric protein that comprises ASC inducer domain and an effector domain.
A kit comprising:
- first and second nucleic acids each encoding a chimeric protein that comprises an ASC inducer domain and an effector domain;
- and
- an ASC inducer compound.
A composition comprising:
- an ASC inducer compound;
- and
- a pharmaceutically acceptable vehicle.
15-16. (canceled)
Dependencies are inferred from the claim text.