US 2013/0157899 A1Published application

Reagents and methods relating to dna assays using amplicon probes on encoded particles

AssigneePerkinElmer Health Sciences, Inc.

InventorsKarl Edwin Adler, JR., Mack J. Schermer

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Abstract

Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.

Claims10

Dependent claims

A method of preparing an encoded bead set for assaying DNA, comprising:

a) performing a first amplification reaction using a DNA template and first reaction oligonucleotide primers, each of the plurality of first reaction primers comprising a variable non-specific degenerate DNA sequence and a contiguous constant DNA sequence, to yield a first reaction product comprising first amplicons, wherein each individual first amplicon comprises a DNA sequence identical to a random portion of the DNA template and a DNA sequence identical to the constant DNA sequence of the first reaction primers;
b) performing a second amplification reaction using at least a portion of the first amplicons as template DNA and second reaction oligonucleotide primers, the second reaction oligonucleotide primers comprising the constant DNA sequence of the first reaction primers, to yield a second reaction product comprising second amplicons, wherein each individual second amplicon comprises a DNA sequence identical to a random portion of the DNA template and a DNA sequence identical to the constant DNA sequence of the first reaction primers;
c) binding the second amplicons to a first plurality of encoded particles, yielding an encoded particle set for assaying DNA.

Dependencies are inferred from the claim text.

Facts

Application: 13/773,089Filed 21 Feb 2013
Priority: 5 Dec 2007
Published: 20 Jun 2013
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6811
Involving nucleic acids: selection methods for production or design of target specific oligonucleotides or binding molecules
C12Q 1/6809
Involving nucleic acids: methods for determination or identification of nucleic acids involving differential detection
C12Q 1/6813
Involving nucleic acids: hybridisation assays
C12Q 1/6876
Involving nucleic acids: nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Family

Application60/992,489Us provisional application · filed 5 Dec 2007
Application12/055,919Continuation · filed 26 Mar 2008
Application13/052,795Division · filed 21 Mar 2011
Application13/773,089Division
Application13/052,795Continuation
US 2009/0148841 A1Published application
US 2011/0172117 A1Published application
US 7,932,037 B2Related patent
US 8,404,463 B2Related patent
US 9,127,307 B2Related patent
US 2013/0157899 A1This patent

From the US filing history. Foreign family members aren’t listed.

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