Universal or broad range assays and multi-tag sample specific diagnostic process using non-optical sequencing
OwnerScot E. Dowdinventor
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Abstract
The present invention includes a method for determining the identify of an organism or virus in a sample comprising the steps of: isolating DNA or RNA from the sample; combining the DNA or RNA directly or with one or more universal or target specific amplification primers, wherein the one or more primers are specific for one or more group of target microorganisms or virus; and amplifying the DNA, or the RNA following reverse transcription with a reverse transcriptase; and contacting the amplification product with one or more species-, organism- or virus-specific detectable marker.
Claims35
A method for determining the identify of an organism or virus in a sample comprising the steps of:
- isolating DNA or RNA from the sample;
- combining the DNA or RNA directly or with one or more universal or target specific amplification primers, wherein the one or more primers are specific for one or more group of target microorganisms or virus;
- amplifying the DNA, or the RNA following reverse transcription with a reverse transcriptase;
- and
- contacting the amplification product with one or more species-, organism- or virus-specific detectable marker.
A method for determining the identify of an organism or virus in a sample comprising the steps of:
- isolating a DNA or RNA from the sample;
- combining the DNA or RNA with one or more universal amplification primers, wherein the one or more primers are specific for one or more target organisms or virus;
- amplifying the DNA, or the RNA following reverse transcription with a reverse transcriptase;
- and
- sequencing the amplified products with a non-optical nucleic acid sequencer.
Dependencies are inferred from the claim text.