Method for Reducing Adapter-Dimer Formation
AssigneeNew England Biolabs, Inc.
InventorsLarry A. McReynolds, Daniela Munafo
Life of this patent
Estimating the term…
Abstract
Methods are provided for ligating a 3′ adapter and a 5′ adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3′ adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3′ adapter and the ligated 3′ adapter. Subsequently, a 5′ adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.
Claims14
A method for ligating a 3′ adapter and a 5′ adapter to a target polynucleotide so as to avoid adapter dimer formation, comprising:
- (a) performing a first ligation between a 3′ end adapter and a target polynucleotide to form a first ligation product;
- (b) adding a blocking oligonucleotide for hybridizing to the 3′ adapter;
- (c) ligating the first ligation product to a 5′ adapter so as to avoid adapter dimer formation.
Dependencies are inferred from the claim text.