Diagnostic detection devices and methods
AssigneeChurch & Dwight Co., Inc.
InventorsAlbert R. Nazareth, Timothy Snowden, Mary-Beth Boyle
Life of this patent
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Abstract
Described herein are methods and devices for detecting the presence of an analyte in a liquid sample. In some embodiments, methods and devices for the detection of an antigen in a body fluid using a plurality of species-specific antibodies are provided. A sandwich complex may be formed using primary antibodies derived from two different species.
Claims64
A device for determining the presence of an analyte in a liquid sample, the device comprising:
- a release medium forming an upstream portion of the device;
- at least two primary antibodies that recognize the analyte that are derived from at least two different species, wherein the at least two primary antibodies are located in or on the release medium;
- a capture medium forming a downstream portion of the device;
- at least one secondary antibody specific for only one of the at least two primary antibodies, wherein the at least one secondary antibody is located in or on the capture medium.
A device for determining the presence of an analyte in a liquid sample, the device comprising:
- a release medium comprising:
- a labeled conjugate comprising a binding member derived from a first species and a label, wherein the binding member is reactive with a first epitope of the analyte;
- and
- a capturable component derived from a second species, wherein the capturable component is reactive with a second epitope of the analyte, such that if the analyte is present in the sample, a sandwich complex is formed comprising the labeled conjugate, the analyte, and the capturable component;
- and
- a capture medium comprising a capture site for capturing the complex, the capture site having immobilized thereon a capture component having specificity for the capturable component.
A method of determining the presence of an analyte in a liquid sample, the method comprising:
- forming a sandwich complex comprising a first primary antibody derived from a first species, the analyte, and a second primary antibody derived from a second, different species;
- and
- capturing the sandwich complex with a secondary antibody specific for only one of the first and second different species.
A method for determining the presence of an analyte in a liquid sample, the method comprising:
- contacting the liquid sample with a release medium comprising:
- a labeled conjugate comprising a binding member derived from a first species, wherein the binding member is reactive with a first epitope of the analyte, and wherein the binding member is labeled with a detectable marker;
- and
- a capturable component derived from a second species, wherein the capturable component is reactive with a second epitope of the analyte, such that if the analyte is present in the sample, a complex comprising the labeled conjugate, the analyte and the capturable component is formed;
- contacting the liquid sample with a capture medium following exposure to the release medium, wherein the capture medium comprises a capture site for capturing the complex, the capture site having immobilized thereon a capture component having specificity for the capturable component;
- and
- determining whether the analyte is present in the liquid sample by observing the presence of the detectable marker at the capture site.
Dependencies are inferred from the claim text.