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US 2013/0157376 A1Published application
Thermal Cycler Calibration Device and Related Methods
AssigneeIdaho Technology, Inc.
InventorLyle Marion Nay
Life of this patent
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Abstract
Methods, devices, and systems are provided for calibrating heat sources of thermal cyclers.
Claims15
A method for calibrating a thermal cycling apparatus having at least one heat source, comprising the steps of:
- providing a container comprising at least one sample vessel, wherein vessel comprises a calibration mixture comprising nucleic acids having at least one nucleic acid melting domain, the melting domain having a predetermined temperature, and a reagent that produces a signal to differentiate between single-stranded and double-stranded nucleic acids,
- introducing the container into the thermal cycling apparatus,
- heating the contents of the container,
- monitoring the reagent to calculate a measured melting temperature, and
- adjusting the heat source to correct discrepancies between the predetermined melting temperature and the measured melting temperature of melting domains.
2 more elements
A device for use in calibrating a thermal cycling apparatus, comprising:
- a container comprising a plurality of sample vessels, each sample vessel comprising a calibration mixture comprising nucleic acids having at least one nucleic acid melting domain, the melting domain having a predetermined temperature, and a reagent that produces a signal to differentiate between single-stranded and double-stranded nucleic acids and configured to generate a measured temperature for the melting domain, wherein the calibration mixture does not contain sufficient components for amplification and the sample vessel is provided sealed to prevent addition of components for amplification
A method for calibrating a thermal cycling apparatus having at least one heat source, comprising the steps of:
- providing a container comprising at least one sample vessel, wherein vessel comprises a calibration mixture comprising temperature-indicating reagent that provides a measurable signal a temperature, the reagent having a predetermined temperature,
- introducing the container into the thermal cycling apparatus,
- heating the contents of the container,
- monitoring the reagent to calculate a measured temperature, and
- adjusting the heat source to correct discrepancies between the predetermined temperature and the measured temperature.
2 more elements
A system for calibration comprising:
- a container comprising at least one sample vessel, wherein vessel comprises a calibration mixture comprising nucleic acids having at least one nucleic acid melting domain, the melting domain having a predetermined temperature, and a reagent that is configured to produce a measured melting temperature of the calibration mixture,
- a thermal cycler system comprising
- at least one heat source, and
- computing device configured to calculate desired adjustment of heat output from the heat source using the predetermined temperature and the measured temperature.
1 more element
Dependencies are inferred from the claim text.