Method for improved transformation using agrobacterium
AssigneeDow AgroSciences LLC
InventorPaul David Miller
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Abstract
Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after exposure to the Agrobacterium cells. Examples of plants useful with these methods include maize plants (e.g., immature maize embryos).
Claims20
A method for plant cell transformation comprising exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant, the surfactant having a concentration of 0.001 weight percent to 0.08 weight percent in the liquid medium.
A method for plant cell transformation comprising:
- preparing a liquid medium containing a surfactant, the surfactant having a concentration of 0.001 weight percent to 0.08 weight percent in the liquid medium;
- suspending Agrobacterium cells in the liquid medium;
- and
- exposing plant cells to the Agrobacterium cells in the liquid medium containing the surfactant.
Dependencies are inferred from the claim text.