Induced pluripotent stem cells prepared from human kidney-derived cells
AssigneeAdvanced Technologies and Regenerative Medicine, LLC
InventorsCharito Buensuceso, Agnieszka Seyda, David C. Colter, Sridevi Dhanaraj, Brian C. Kramer, Jason Elliot Ekert, Amanda Lynn Kauffman
Life of this patent
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Abstract
We have disclosed an induced pluripotent stem cell and the method of preparing the induced pluripotent stem cell from a human kidney-derived cell. More particularly, we have disclosed a human kidney-derived iPS cell which may be differentiated into cells of ectoderm, mesoderm, and endoderm lineages.
Claims6
-5. (canceled)
A method of making a human kidney-derived induced pluripotent cell comprising the steps of:
- providing a human kidney-derived cell, wherein the human kidney-derived cell is positive for the expression of HLA-I and CD 44 and at least one of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, EpoR, BMP2, BMP7, or GDF5;
- and negative for the expression of CD133 and E-cadherin and at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 or GATA-4, transfecting the human kidney derived-cell with each one of a VSVg murine retrovirus expressing human transcription factor OCT4, a VSVg murine retrovirus expressing human transcription factor SOX2, a VSVg murine retrovirus expressing human transcription factor KLF4, and a VSVg murine retrovirus expressing human transcription factor c-MYC,
- culturing the transfected human kidney-derived cell,
- identifying an induced pluripotent stem cell,
- isolating the induced pluripotent stem cell,
- subculturing the induced pluripotent stem cell, and
- providing an induced pluripotent stem cell.
A method of making a human kidney-derived induced pluripotent cell comprising the steps of:
- providing a human kidney-derived cell, transfecting the human kidney-derived cells with each one of an mRNA encoding a Oct-4 protein, an mRNA encoding a Sox2 protein, an mRNA encoding a Klf4 protein, an mRNA encoding a c-myc protein, and an mRNA encoding a Lin28 protein,
- culturing the transfected human kidney-derived iPS cell,
- identifying a induced pluripotent stem cell,
- isolating the induced pluripotent stem cell,
- subculturing the induced pluripotent stem cell, and
- providing an induced pluripotent stem cell.
Dependencies are inferred from the claim text.