Induced pluripotent stem cells from human umbilical cord tissue-derived cells
AssigneeAdvanced Technologies and Regenerative Medicine, LLC
InventorsCharito Buensuceso, Agnieszka Seyda, David C. Colter, Sridevi Dhanaraj, Brian C. Kramer
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Abstract
We have disclosed an induced pluripotent stem cell and the method of preparing the induced pluripotent stem cell from a human umbilical cord tissue-derived cell. More particularly, we have disclosed a human umbilical cord tissue-derived iPS cell which may be differentiated into cells of ectoderm, mesoderm, and endoderm lineages.
Claims7
An induced pluripotent stem cell comprising a reprogrammed human umbilical cord tissue-derived cell wherein the human umbilical cord tissue-derived cell is an isolated umbilical cord tissue cell isolated from human umbilical cord tissue substantially free of blood that is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, can undergo at least 40 doublings in culture, maintains a normal karyotype upon passaging, and has the following characteristics:
- expresses each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, and HLA-A,B,C;
- does not express any of CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G, or HLA-DR,DP,DQ;
- and increased expression of a gene for each of interleukin 8;
- reticulon 1;
- and chemokine (C-X-C motif) ligand 3 relative to that of a human cell which is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell.
An induced pluripotent stem cell prepared by a method comprising the steps of:
- providing a human umbilical cord tissue-derived cell, wherein the human umbilical cord tissue-derived cell is an isolated umbilical cord tissue cell isolated from human umbilical cord tissue substantially free of blood that is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, can undergo at least 40 doublings in culture, maintains a normal karyotype upon passaging, and has the following characteristics:
- expresses each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, and HLA-A,B,C;
- does not express any of CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G, or HLA-DR,DP,DQ;
- and increased expression of a gene for each of interleukin 8;
- reticulon 1;
- and chemokine (C-X-C motif) ligand 3 relative to that of a human cell which is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell;
- transfecting the human umbilical cord tissue derived-cell with murine retroviruses, individually carrying constitutively expressed human transcription factors OCT4, SOX2, KLF4, and c-MYC,
- culturing the transfected human umbilical cord tissue-derived cell, identifying an induced pluripotent stem cell,
- isolating the human umbilical cord tissue-derived IPS cell, subculturing the induced pluripotent stem cell, and
- providing a induced pluripotent stem cell.
Dependencies are inferred from the claim text.