US 2013/0157364 A1 · MEDIUM COMPOSITION FOR CULTURING SELF-ACTIVATED LYMPHOCYTES AND METHOD FOR CULTURING SELF-ACTIVATED LYMPHOCYTES USING SAME

Abstract

Disclosed is a medium composition for culturing self-activated lymphocytes, which contains anti-CD3 antibody and anti-CD16 antibody in addition to interleukin 2 (IL-2), interleukin 12 (IL-12) and interleukin 18 (IL-18) in a medium, and thus can efficiently proliferate and activate NK cells, T cells and NKT cells and, at the same time, can significantly increase the ratio of NK cells in lymphocytes so as to provide immunocytes having excellent effects on the treatment of various kinds of malignant tumors, and a method for culturing self-activated lymphocytes using the medium composition.

Claims14

Dependent claims

A medium composition for culturing self-activated lymphocytes on a large scale, the medium composition comprising:

a cell culture medium;
and additives that are added to the cell culture medium, wherein the additives include interleukin-2 (IL-2), interleukin 12 (IL-12), interleukin 18 (IL-18), anti-CD3 antibody and anti-CD16 antibody.

A method for culturing self-activated lymphocytes, the method comprising the steps of:

extracting lymphocytes from human peripheral blood;
culturing the extracted lymphocytes in a medium containing interleukin 2 (IL-2), L-glutamine and autologous plasma in the presence of interleukin 12 (IL-12), interleukin 18 (IL-18), anti-CD3 antibody and anti-CD16 antibody (first culture step);
and
adding the lymphocyte-containing medium resulting from the first culture step to a medium containing IL-2, L-glutamine and autologous plasma, followed by culture in the presence of IL-12, anti-CD16 antibody and IL-18 (second culture step).

1 more element

Dependencies are inferred from the claim text.

Facts

Application

13/819,426Filed 1 Jun 2011

Priority

KR 10-2010-008383630 Aug 2010

Published

20 Jun 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12N 5/0636
Cells from the blood or the immune system: t lymphocytes
A61K 40/11
Characterised by the cell type used: t-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer…
A61K 40/15
Characterised by the cell type used: natural-killer [NK] cells; Natural-killer T [NKT] cells
A61K 40/42
Vertebrate antigens: cancer antigens

Medium composition for culturing self-activated lymphocytes and method for culturing self-activated lymphocytes using same

Life of this patent

Medium composition for culturing self-activated lymphocytes and method for culturing self-activated lymphocytes using same

Life of this patent

Abstract

Claims14